Analysis Note

Negative ControlHPB-MLT

Positive Control4937 cells

Application

Flow Cytometry (10 µg/ml)r>Frozen Sections (see comments)r>Immunoblotting (see comments)r>Immunoprecipitation (see comments)r>Neutralization Studies (see comments)

General description

Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.

Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Recognizes the ~72 kDa CD64 protein (FcR1 receptor).

Immunogen

Human

human rheumatoid synovial fluid cells/monocytes

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵ cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)